Thermo Fisher Scientific SNAP25 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Mouse, Rat, Zebrafish

Published species

Chicken, Guinea pig, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide:(C)- A(195) N Q R A T K M L G S G(206) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SNAP25,uniProtId:P60879-1,ncbiNodeId:10090,antigenRange:195-206,antigenLength:206,antigenImageFileName:PA1-740_SNAP25_P60879-1_Rabbit.svg,antigenImageFileNamePDP:PA1-740_SNAP25_P60879-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2192212

Product Specific Information

PA1-740 detects synaptosomal associated protein 25 (SNAP-25) from bovine, mouse, rat and zebra fish (Danio rerio) samples.

PA1-740 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 25 kDa protein representing SNAP-25 in whole rat brain, mouse brain and zebra fish extracts. Immunofluorescence staining of SNAP-25 in cultured rat hippocampal cells with PA1-740 yields a pattern consistent with cytoplasmic and plasma membrane staining.

PA1-740 immunizing peptide corresponds to the C-terminal amino acid residues 195-206 of mouse SNAP-25. This sequence is completely conserved between mouse, rat, human, chicken, and goldfish. PA1-740 immunizing peptide (Cat. # PEP-032) is available for use in neutralization and control experiments.

Target Information

SNAP25 is a t-SNARE involved in the molecular regulation of neurotransmitter release. It may play a role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. It also regulates plasma membrane recycling through its interaction with CENPF. Mutations in the gene can results in myasthenic syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.