Thermo Fisher Scientific SNAP25 Polyclonal Antibody
SNAP25 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 높은 특이성과 재현성을 제공하며, 신경전달 관련 연구에 적합. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Thermo Fisher Scientific SNAP25 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 2 publications |
| Neutralization (Neu) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Mouse, Rat, Zebrafish |
| Published Species | Chicken, Guinea Pig, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic Peptide: (C)-A(195)NQRATKMLGSG(206) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2192212 |
Product Specific Information
PA1-740 detects synaptosomal associated protein 25 (SNAP-25) from bovine, mouse, rat, and zebrafish samples.
It has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 25 kDa protein representing SNAP-25 in whole rat brain, mouse brain, and zebrafish extracts.
Immunofluorescence staining of SNAP-25 in cultured rat hippocampal cells yields a pattern consistent with cytoplasmic and plasma membrane localization.
PA1-740 immunizing peptide corresponds to the C-terminal amino acid residues 195–206 of mouse SNAP-25. This sequence is completely conserved among mouse, rat, human, chicken, and goldfish.
The immunizing peptide (Cat. # PEP-032) is available for use in neutralization and control experiments.
Target Information
SNAP25 is a t-SNARE involved in the molecular regulation of neurotransmitter release.
It plays a role in the synaptic function of specific neuronal systems and associates with proteins involved in vesicle docking and membrane fusion.
Additionally, it regulates plasma membrane recycling through interaction with CENPF.
Mutations in this gene can result in myasthenic syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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