Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Violet 605

Applications

• Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

Description:
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 1.0 µg/test (100 µL final volume). Cell count may range from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal assay performance.

Fluorochrome Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm and is designed for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV605 is not compatible with methanol-based fixation.

Excitation / Emission / Laser:
407 nm / 605 nm / Violet Laser.

Trademark Notice:
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen):
A receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. Regulates Src-family kinases, lymphocyte development, and antigen signaling. Exists as multiple isoforms via alternative splicing, differing in extracellular domains. Also involved in integrin-mediated signaling, cell survival, and apoptosis-related DNA fragmentation. CD45RA is an isoform with cell-type restricted expression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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