Thermo Fisher Scientific CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test
• Tested and validated for flow cytometric analysis of normal human peripheral blood cells.
• Recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright conjugates.

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• Super Bright 436
• Custom conjugation available upon request

Product Specific Information

The eBioSY11B5 monoclonal antibody recognizes human CD157 (also known as Mo5 or BST-1), a 42–45 kDa GPI-anchored protein structurally and functionally related to CD38. CD157 is expressed on monocytes, macrophages, and bone marrow stromal cells, and is involved in NAD⁺ metabolism and intracellular Ca²⁺ regulation. It can act both as an enzyme and as a receptor initiating signal transduction cascades involving phosphorylation of cytoplasmic proteins such as focal adhesion kinase (FAK).

Applications Reported:

• Flow cytometric analysis of human peripheral blood cells.
• Use at 5 µL (0.25 µg) per test (100 µL final staining volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:

• Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm.
• Recommended detection filter: 610/20 bandpass.
• Protect from light due to photo-induced oxidation sensitivity.

Fixation Guidance:

• Samples can be stored up to 3 days in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) at 4°C in the dark with minimal impact on brightness and compensation.

Laser Compatibility:

• Excitation: 405 nm
• Emission: 600 nm
• Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Bone marrow stromal cell antigen 1 (BST1) is a glycosylphosphatidylinositol-anchored protein facilitating pre-B cell growth. Also known as ADP-ribosyl cyclase 2 or DAMP-1, BST1 expression is enhanced in bone marrow stromal cells from rheumatoid arthritis patients, possibly contributing to abnormal B cell activation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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