Thermo Fisher Scientific CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), Super Bright 600, eBioscience
인간 CD157(BST-1)을 인식하는 마우스 단클론 항체로, Super Bright 600 형광체로 결합되어 유세포분석(Flow Cytometry)에 최적화됨. 405 nm 레이저에서 여기, 600 nm에서 방출. 비특이적 결합 최소화를 위해 Super Bright Staining Buffer 사용 권장. 연구용 전용.
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Applications
- Flow Cytometry (Flow): 5 µL (0.25 µg)/test
- Tested and validated for flow cytometric analysis of normal human peripheral blood cells.
- Recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright conjugates.
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSY11B5 (SY11B5) |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL / Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762550 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Yellow 590
- NovaFluor Yellow 610
- PE
- PE-Cyanine7
- Super Bright 436
- Custom conjugation available upon request
Product Specific Information
The eBioSY11B5 monoclonal antibody recognizes human CD157 (also known as Mo5 or BST-1), a 42–45 kDa GPI-anchored protein structurally and functionally related to CD38. CD157 is expressed on monocytes, macrophages, and bone marrow stromal cells, and is involved in NAD⁺ metabolism and intracellular Ca²⁺ regulation. It can act both as an enzyme and as a receptor initiating signal transduction cascades involving phosphorylation of cytoplasmic proteins such as focal adhesion kinase (FAK).
Applications Reported:
- Flow cytometric analysis of human peripheral blood cells.
- Use at 5 µL (0.25 µg) per test (100 µL final staining volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
- Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm.
- Recommended detection filter: 610/20 bandpass.
- Protect from light due to photo-induced oxidation sensitivity.
Fixation Guidance:
- Samples can be stored up to 3 days in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) at 4°C in the dark with minimal impact on brightness and compensation.
Laser Compatibility:
- Excitation: 405 nm
- Emission: 600 nm
- Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Bone marrow stromal cell antigen 1 (BST1) is a glycosylphosphatidylinositol-anchored protein facilitating pre-B cell growth. Also known as ADP-ribosyl cyclase 2 or DAMP-1, BST1 expression is enhanced in bone marrow stromal cells from rheumatoid arthritis patients, possibly contributing to abnormal B cell activation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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