Thermo Fisher Scientific ATM Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:1,000

Product Specifications

Product Specific Information

Purity is greater than 95% by SDS-PAGE.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer. The ATM gene encodes a protein in the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins rather than lipids and has several downstream targets acting as cell-cycle regulators, including P53, Mdm2, BRCA1, and SMC1.

ATM is responsible for repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens. The C-terminal region of ATM shares homology with the catalytic domains of PI3 kinases. Studies show that ATM becomes autophosphorylated and upregulated upon exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in DNA synthesis inhibition, and show delayed p53 induction. DNA damage activates ATM kinase, leading to a cascade of kinase reactions regulating cell cycle, apoptosis, and DNA damage repair. ATM is also linked to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.