Thermo Fisher Scientific MAP1A/MAP1B LC3 A/B Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome or vacuole for degradation. During macroautophagy, these vesicles, termed autophagosomes, fuse with the lysosome, resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment.

There are 16 proteins participating in the autophagy pathway in humans. LC3, the mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both MAP1A and MAP1B microtubule-binding domains. LC3-I is cytosolic, while LC3-II is membrane-bound and enriched in the autophagic vacuole fraction. The LC3-I to LC3-II conversion serves as a sensitive marker for autophagy detection in mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.