Thermo Fisher Scientific Green Renilla luciferase Polyclonal Antibody
Green Renilla luciferase 단백질을 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, IP, Neutralization 등 다양한 응용에 사용 가능. 높은 특이성과 감도를 제공하며, 비방사성 루시퍼레이스 분석에 적합. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:4,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 | - |
| Immunoprecipitation (IP) | 5 µg | - |
| Neutralization (Neu) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Renilla |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified full length recombinant Green Renilla luciferase expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 30% glycerol, 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539911 |
Product Specific Information
Western blot analysis of PA1-180 detects a prominent ~36 kDa protein in Green Renilla luciferase expressing cells. Additional nonspecific bands may appear in control cells with increased exposure time.
This antibody has been successfully tested in IP, immunofluorescence, and neutralization assays.
Green Renilla luciferase activity can be measured using Thermo Fisher Firefly Luciferase Flash or Glow Assay Kits, or Luciferase Dual Assay Kits.
Target Information
The Green Renilla luciferase is a 36 kDa protein derived from the wild-type Renilla reniformis gene (sea pansy). Compared to wild-type luciferase, Green Renilla is more stable in serum and exhibits an emission spectrum shifted toward the green region.
It provides an extremely bright flash signal that decays rapidly. The Renilla Flash and Glow Assay Kit reagents allow for Glow (stabilized signal with ~3-hour half-life) or Flash-type bioluminescence experiments and are optimized for use with Thermo Scientific Green Renilla Luc Plasmids.
Bioluminescence enables non-radioactive assays for measuring transcriptional activity of regulatory elements, promoter activity studies, upstream pathway analysis, and evaluation of activators or inhibitors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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