Thermo Fisher Scientific Green Renilla luciferase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:4,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500-1:1,000

-

Immunoprecipitation (IP)

5 µg

-

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Species Reactivity

Renilla

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified full length recombinant Green Renilla luciferase expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539911

Product Specific Information

Western blot analysis of PA1-180 detects a prominent ~36 kDa protein in Green Renilla luciferase expressing cells. Additional unspecific bands are also detected in control cells with increased exposure time. This antibody has also been successfully tested in IP, Immunofluorescence, and neutralization assays.

Green Renilla luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash or Glow Assay Kits or Luciferase Dual Assay Kits.

Target Information

The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Renilla Flash and Glow Assay Kit reagents allow for Glow (stabilized signal with ~3 hours halflife) or Flash type bioluminescence experiments and are optimized for best results with Thermo Scientific Green Renilla Luc Plasmids. Bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.