Thermo Fisher Scientific Cytokeratin 10/13 Monoclonal Antibody (SPM262)
Cytokeratin 10/13 단백질을 인식하는 Mouse monoclonal antibody로, Human 및 Cat 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능하며, Protein A/G로 정제된 200 µg/mL 액상 형태입니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Cat, Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM262 |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells found in both keratinizing tissues (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells). Although not typical markers for endothelial cells, cytokeratins can also be present in some microvascular endothelial cells.
At least 20 different CKs, ranging from 40–70 kDa with isoelectric points of 5–8.05, can be identified using two-dimensional gel electrophoresis.
Biochemically, CKs are classified into two groups: type I (acidic polypeptides) and type II (basic polypeptides). Both types are expressed in all epithelial cells.
Monoclonal antibodies to cytokeratin proteins are valuable tools for tumor identification and classification.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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