Thermo Fisher Scientific Acrolein Monoclonal Antibody (10A10)
Acrolein 변형 단백질을 특이적으로 검출하는 Mouse IgG1 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. KLH 기반 합성 항원으로 제작되었으며, 단백질 G로 정제된 액상 형태. -20°C 보관, 연구용 전용.
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Thermo Fisher Scientific Acrolein Monoclonal Antibody (10A10)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View publication |
| Immunocytochemistry (ICC/IF) | 1:50 | View publication |
| Flow Cytometry (Flow) | 1:50 | - |
| ELISA | 1:1,000 | - |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A10 |
| Immunogen | Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735099 |
Available Formats
Product Specific Information
A 1:1,000 dilution of MA5-27553 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Acrolein modified proteins. Does not detect free acrolein.
Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. It may be formed from the breakdown of certain pollutants in outdoor air or from the burning of organic matter including tobacco or fuels such as gasoline or oil. Acrolein is toxic to humans following inhalation, oral, or dermal exposures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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