Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7)
HDEL 모노클론 항체(2E7)는 효모, 식물, 초파리 등에서 HDEL 서열을 인식하는 항체로, 단백질의 ER 보유 신호 검출에 적합합니다. Western blot 및 ICC/IF에 사용 가능하며, 고순도 Protein G 정제 및 안정한 PBS/glycerol 저장 버퍼를 사용합니다.
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Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Plant, Yeast |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Immunogen | Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735212 |
Available Formats
Product Specific Information
1 µg/mL of MA5-27549 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78 kDa.
Target Information
HSP 70 family comprises four highly conserved proteins: HSP 70, HSC 70, GRP 75, and GRP 78. These proteins act as molecular chaperones, assisting in multi-protein complex assembly, translocation across membranes, and proper folding of nascent chains.
GRP 78 is localized in the endoplasmic reticulum (ER), where it aids in folding and translocation of secretory proteins. Sorting of these proteins depends on a C-terminal tetrapeptide signal—KDEL in animal cells and HDEL in S. cerevisiae.
Clone 2E7 recognizes the C-terminal peptide HDEL, a common ER retention signal found in yeast, plants, nematodes, and other eukaryotic proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum, and wheat.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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