Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9)
Hexanoyl-Lysine adduct에 특이적인 Mouse monoclonal antibody (Clone 5D9). Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용 가능. HEL 변형 단백질 검출용으로, 다른 알데하이드 변형 단백질과 교차 반응 없음. 연구용으로만 사용.
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Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D9 |
| Immunogen | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735214 |
Available Formats
- APC conjugate: MA5-45777
- FITC conjugate: MA5-45778
- PE conjugate: MA5-45780
- PerCP conjugate: MA5-45779
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-27554 was sufficient for detection of Hexanoyl-Lysine adduct in 0.5 µg of Hexanoyl-Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine.
No cross-reactivity with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker derived from the oxidation of omega-6 unsaturated fatty acids. HEL may serve as a biomarker for omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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