Thermo Fisher Scientific Phospho-HSP27 (Ser82) Recombinant Rabbit Monoclonal Antibody (SN06-73)
Phospho-HSP27 (Ser82) 표적에 특이적인 Thermo Fisher recombinant rabbit monoclonal antibody로 높은 특이성과 재현성을 제공. Western blot에 최적화된 1:1,000–1:2,000 희석 비율. 인간, 마우스, 랫트 반응성. Protein A 정제 및 TBS buffer 저장. 연구용 전용 제품.
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Applications
- Western Blot (WB): 1:1,000–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN06-73 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser82 of Human Hsp27 (aa 50–99) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809552 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select optimal candidates for production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Cells respond to environmental stress by increasing expression of heat shock or stress proteins. HSPB1 (HSP27) is a small heat shock protein functioning as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It contributes to stress resistance and actin organization, and regulates processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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