Thermo Fisher Scientific Human Lactoferrin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Goats were immunized with purified human lactoferrin from human milk. The antiserum was solid-phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient (absorbance at 280 nm of 1.4 equals 1.0 mg of IgG).

Target Information

Lactoferrin (LF) is an iron-binding glycoprotein composed of a single polypeptide chain (~80 kDa) folded into two homologous lobes, each capable of reversibly binding one ferric ion (Fe³⁺). LF is present in external fluids such as milk and mucosal secretions and is a major component of secondary granules of neutrophils.
It plays key roles in:

• Regulation of iron homeostasis
• Host defense against microbial infections
• Anti-inflammatory activity
• Regulation of cellular growth and differentiation
• Protection against cancer development and metastasis

Lactoferrin acts as a first-line defense against microbial infections by binding and removing Fe³⁺ from the environment and exhibits bactericidal and proteolytic activities, capable of cleaving arginine-rich sequences in microbial proteins.
Three isoforms of LTF (α, β, γ) have been identified, and receptors are expressed in the gastrointestinal tract, macrophages, lymphocytes, neutrophils, platelets, and some bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.