Thermo Fisher Scientific Human Lactoferrin Polyclonal Antibody, Biotin

Applications

Western Blot (WB)

• Tested Dilution: 1:10,000 - 1:100,000

ELISA

• Tested Dilution: 1:10,000 - 1:100,000

Product Specifications

Product Specific Information

Goats were immunized with purified human lactoferrin from human milk. Antiserum was solid-phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Target Information

Lactoferrin (LF) is an iron-binding glycoprotein composed of a single polypeptide chain (~80 kDa) folded into two homologous lobes, each capable of reversibly binding one ferric ion (Fe³⁺). LF is present in external fluids such as milk and mucosal secretions and is abundant in neutrophil secondary granules.

This protein plays diverse biological roles, including:

• Regulation of iron homeostasis
• Host defense against microbial infections
• Anti-inflammatory activity
• Control of cellular growth and differentiation
• Protection against cancer development and metastasis

Lactoferrin acts as a major first line of defense against microbial infection by binding Fe³⁺ and depriving pathogens of iron. It also exhibits bactericidal and proteolytic activities, cleaving arginine-rich sequences in microbial proteins. Three isoforms (LTF-α, LTF-β, LTF-γ) have been identified, and LTF receptors are expressed in the gastrointestinal tract, macrophages, lymphocytes, neutrophils, platelets, and some bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.