Thermo Fisher Scientific PARP1 (cleaved Asp214) Polyclonal Antibody
PARP1 (cleaved Asp214) 특이적 폴리클로날 항체로, 인간 시료에서 Western blot 및 면역세포염색(ICC/IF)에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1.33 mg/mL 농도의 액상 형태로 제공됩니다. 세포사멸 연구 및 PARP 단백질 절단 검출에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PARP1 (cleaved Asp214). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.33 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736324 |
Product Specific Information
- Positive Control: 293T, HCT116, HCT116 (30 µM cisplatin treatment for 24 hr)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form, NAD) as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a site conserved across species.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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