Thermo Fisher Scientific ATP6V1A Polyclonal Antibody
ATP6V1A 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 Immunocytochemistry에 적합. 인간 시료에 반응하며 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보관, 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human V-ATPase A (Recombinant protein control fragment, Product #RP-104654) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2662834 |
Product Specific Information
Immunogen sequence:
YVHGVSGPVVT ACDMAGAAMY ELVRVGHSEL VGEIIRLEGD MATIQVYEET SGVSVGDPVL RTGKPLSVEL GPGIMGAIFD GIQRPLSDIS SQT
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for intracellular processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase is composed of:
- V1 domain (cytosolic): three A and three B subunits, two G subunits, plus C, D, E, F, and H subunits (contains ATP catalytic site)
- V0 domain (transmembrane): five subunits (a, c, c’, c”, and d)
Additional isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is one of two V1 domain A subunit isoforms and is expressed in all tissues.
Transcript variants derived from alternative polyadenylation exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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