Thermo Fisher Scientific ATP6V1A Polyclonal Antibody
ATP6V1A 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 적합하며, Human 시료 반응성. 항원 친화 크로마토그래피로 정제, PBS/glycerol buffer에 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human V-ATPase A. Recombinant protein control fragment (Product # RP-104653) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2662833 |
Product Specific Information
Immunogen sequence:
AIPGAFGCGKT VISQSLSKYS NSDVIIYVGC GERGNEMSEV LRDFPELTME VDGKVESIMK RTALVANTSN MPVAAREASI YTGITL
Highest antigen sequence identity to the following orthologs: mouse 100%, rat 100%.
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
V-ATPase comprises:
- V1 domain (cytosolic): three A and three B subunits, two G subunits, and C, D, E, F, H subunits. Contains the ATP catalytic site.
- V0 domain (transmembrane): five subunits (a, c, c', c'', d).
Additional isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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