Thermo Fisher Scientific Nitrotyrosine Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:8,000

ELISA

• Tested Dilution: 1:1,000–1:8,000

Product Specifications

Target Information

Nitrotyrosine is a relatively stable product formed from various reaction pathways. The most notable is the reaction of peroxynitrite (formed from superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins, resulting in inactivation of certain housekeeping enzymes (e.g., alpha1-antiproteinase) and endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD).

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of nitric oxide (NO) production. Measuring nitrotyrosine concentration serves as a marker for NO-induced cellular damage. It is implicated in the pathogenesis of several inflammatory, infectious, and degenerative human diseases, including Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and conditions associated with endothelial injury.

At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for the oncogenic protein p53 and the mitochondrial protein manganese-dependent superoxide dismutase (MnSOD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.