Thermo Scientific™

LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay Kit, rabbit

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4894:The LanthaScreen™ TR-FRET PPAR gamma Competitive자세히 알아보기

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4894:

The LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor-gamma (PPAR gamma). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and human PPAR gamma ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET PPAR gamma Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR gamma-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR gamma, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR gamma is detected by a test compounds ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous,addition-only` format.

사양

색상Green

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량400 x 40 μL assays

포장384-well Plate

어세이 카테고리Biochemical Assay

약효를 기대할 수 있는 타겟Nuclear Receptors

판독TR-FRET (Binding)

제품라인LanthaScreen™

용도(애플리케이션)Nuclear Receptor Assay, TR-FRET

리간드Pan-PPAR

제품 유형TR-FRET PPAR Gamma Competitive Binding Assay Kit

No. of Assays400 x 40 μL Assays

Unit SizeEach