Thermo Fisher Scientific LanthaScreen TR-FRET PPAR alpha Coactivator Assay Kit, rabbit
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
A15133 | - | Thermo Fisher Scientific A15133 LanthaScreen TR-FRET PPAR alpha Coactivator Assay Kit, rabbit Each pk | 재고문의 | pk | 2,966,000원 | - | 3,262,600원 |
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Thermo Scientific™
LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay Kit, rabbit
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4684:The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator자세히 알아보기
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4684:
The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
Agonist mode:
When running the LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay in agonist mode (to identify agonist compounds), PPAR alpha- LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).
Antagonist mode:
When the LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR alpha-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluorescein-coactivator peptide, and Tb-labeled anti-GST antibody (Figure 2). The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode.
Contents and Storage:
The LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay Kit contains PPAR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or 4°C).
사양
색상Green
콘주게이트Tb (Terbium)
검출 방법Fluorescence
용도(장비)Microplate Reader
수량800 x 20 μL assays
포장384-well Plate
어세이 카테고리Biochemical Assay
약효를 기대할 수 있는 타겟Nuclear Receptors
판독TR-FRET (Activity)
제품라인LanthaScreen™
용도(애플리케이션)Nuclear Receptor Assay, TR-FRET
리간드PPAR alpha
제품 유형TR-FRET PPAR Alpha Coactivator Assay Kit
No. of Assays800 x 20 μL Assays
Unit SizeEach
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