Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 605

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450, 506, 660)
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE and PE-Cyanine series
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all stages of development except terminally differentiated plasma cells. Follicular dendritic cells also express CD19.

CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, associating with the BCR to induce tyrosine phosphorylation, calcium flux, and B-cell proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤0.5 µg/test, 100 µL final volume)

Brilliant Violet™ 605 (BV605) emits at 605 nm and is intended for use with a 405 nm violet laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light
Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution (store up to 3 days at 4°C in dark). Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells and certain myeloid leukemia cells. It serves as a key regulator of B cell activation and proliferation, lowering the threshold for antigen receptor-dependent stimulation.

Mutation of CD19 leads to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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