Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Brilliant Violet 605, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 711
• eFluor 450
• FITC
• NovaFluor Blue / Red / UV / Violet / Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available upon request

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and most mature T cells, and is upregulated upon T-cell stimulation. It binds to CD70 and plays an important role in T cell-B cell interaction.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test in a final volume of 100 µL (cell number: 10⁵–10⁸ cells/test).

Fluorophore Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm and is suitable for cytometers equipped with a 405 nm violet laser. Protect from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Fixation Guidelines:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on fluorescence. BV605 is not compatible with methanol-based fixation.

Excitation/Emission: 407 nm / 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily (including CD40 and CD30). It regulates cell proliferation and death and is predominantly expressed on lymphocytes. CD27 and CD70 expression are key in immune activation and co-stimulation. Soluble CD27 (sCD27) serves as a prognostic marker in B cell malignancies. CD27 dysfunction is associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

제품 이미지