Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

Unconjugated, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–786), eFluor series (450–660), FITC, Functional Grade, NovaFluor series (Blue, Red, Violet, Yellow), PE, PE-Cyanine series, PerCP-Cyanine5.5, Super Bright series (436–780), Custom conjugation available.

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except plasma cells. CD19 forms a complex with CD21, CD81, and MHC class II, associated with the BCR, and is involved in tyrosine phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes; ≤0.5 µg/test (100 µL final volume)
• Recommended: Titrate for optimal performance
• Fluorochrome: Brilliant Violet™ 605 (BV605), emission at 605 nm, suitable for 405 nm laser

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when using multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in dark. Not compatible with methanol-based fixation.
Laser: Violet (407 nm excitation / 605 nm emission).

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating B cell proliferation and activation. Mutations can result in hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.

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