Thermo Fisher Scientific ADAP Polyclonal Antibody
ADAP 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태입니다. 인간 시료에 반응하며, 세포 부착 및 T세포 활성화 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 17 amino acid peptide from near the amino terminus of human ADAP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154458 |
Product Specific Information
- Suggested positive control: K562 cell lysate
- PA5-20401 can be used with blocking peptide PEP-0518
Target Information
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion.
TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappa-B family of transcription factors.
Recent studies have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell–antigen presenting cell (APC) activation.
At least three isoforms of ADAP are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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