Thermo Fisher Scientific LRRC25 Monoclonal Antibody (OTI2A3), TrueMAB
Human LRRC25 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, 고순도 친화 크로마토그래피 정제. RLR 및 NF-κB 신호 억제 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A3 |
| Immunogen | Full length human recombinant protein of human LRRC25 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
LRRC25 plays a role in the inhibition of RLR-mediated type I interferon signaling by targeting DDX58/RIG-I for autophagic degradation.
It interacts with ISG15-associated DDX58 to promote interaction with p62/SQSTM1, mediating selective autophagy of DDX58 (PubMed:29288164).
Also involved in NF-κB signaling inhibition by promoting degradation of p65/RELA. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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