Thermo Fisher Scientific PichiaPink Secretion Signal Set

Invitrogen™ PichiaPink™ Secretion Signal Set

The PichiaPink™ Secretion Signal Set is a collection of 8 DNA fragments encoding secretion signal sequences designed for use with the pPINK-HC and pPINK-LC vectors. These sequences enable efficient secretion of recombinant proteins expressed in Pichia pastoris.

Optimize Secretion of Your Expressed Protein

By adding one of the 8 secretion signal sequences to your gene, your expressed protein can be secreted into the culture medium, simplifying harvesting and purification. Since Pichia pastoris secretes few native proteins, the majority of secreted protein will be your target protein.

Each DNA fragment encodes a different secretion signal, allowing you to test and identify the most effective one for your specific protein.

Included Secretion Signal Sequences

• α-mating factor pre-sequence from Saccharomyces cerevisiae
• α-amylase signal sequence from Aspergillus niger
• Glucoamylase signal sequence from Aspergillus awamori
• Serum albumin signal sequence from Homo sapiens
• Inulinase signal sequence from Kluyveromyces marxianus
• Invertase signal sequence from Saccharomyces cerevisiae
• Killer protein signal sequence from Saccharomyces cerevisiae
• Lysozyme signal sequence from Gallus gallus

Easy Cloning and Expression

Each DNA fragment is designed for easy ligation in front of your gene in either the pPINK-HC or pPINK-LC vector. All sequences include the Kozak sequence from the AOX1 gene of Pichia pastoris to ensure efficient translation.

The secretion signal sequences are supplied as phosphorylated duplex oligomers in 40 pmol aliquots. Resuspend each oligomer in 40 μL TE buffer before use.

Why Choose the PichiaPink™ Yeast Expression System?

The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris, known for:

• Rapid growth and simple media requirements
• Well-characterized genetic background
• Ease of handling and scalability
• Proven success in expressing diverse recombinant proteins

For over 30 years, Pichia pastoris has been widely used for heterologous protein production across various species, including human proteins.

For commercial-use licensing of the PichiaPink™ Yeast Expression System, please contact: outlicensing@lifetech.com

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000;24(1):45–66.
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002;13(4):329–32.

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