Thermo Fisher Scientific Mu-Calpain Polyclonal Antibody
Rabbit polyclonal antibody against human Mu-Calpain (CAPN1). Validated for WB, IHC(P), and ICC/IF. High specificity and affinity purified. Suitable for research use in calpain-related pathways and protein expression studies.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CAPN1 (Product #RP-101847) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789392 |
Product Specific Information
Immunogen sequence:
PDALKSRTIR KWNTTLYEGT WRRGSTAGGC RNYPATFWVN PQFKIRLDET DDPDDYGDRE SGCSFVLALM QKHRRRERRF GRDMETIGFA VYEVPPELVG QPAVHLKRDF FLANASRARS EQFINLREVS TRFRLPPGEY VVVPSTFEPN KE
Target Information
The calpains, including Calpain 1 (CAPN1), are calcium-activated neutral proteases that function as nonlysosomal, intracellular cysteine proteases. Mammalian calpains include ubiquitous, stomach-specific, and muscle-specific isoforms. The ubiquitous enzymes are heterodimers composed of distinct large catalytic subunits and a common small regulatory subunit.
The CAPN1 gene encodes the large subunit of the ubiquitous enzyme Calpain 1, with multiple transcript variants encoding different isoforms.
Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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