Thermo Fisher Scientific P-Selectin Polyclonal Antibody
Human P-selectin을 인식하는 Rabbit Polyclonal Antibody로 Western blot 및 IHC(Paraffin) 검증됨. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 염증 및 혈소판 활성화 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SELP. Recombinant protein control fragment (Product #RP-99385) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789391 |
Product Specific Information
Immunogen sequence:
GSLDCSDTRG EFNVGSTCHF SCDNGFKLEG PNNVECTTSG RWSATPPTCK GIASLPTPGV QCPALTTPGQ GTMYCRHHPG TFGFNTTCYF GCNAGFTLIG DSTLSCRPSG QWTAVTPACR AVKCSELHVN KPIAMNCSNL W
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein involved in cell rolling regulation. It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets, and rapidly translocates to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
During platelet activation and degranulation, P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes. It functions as a calcium-dependent receptor binding sialylated Lewis blood group carbohydrate antigens on neutrophils and monocytes.
P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis, and tissue destruction. Clinically, it is used to distinguish heparin-induced thrombocytopenia with or without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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