Thermo Fisher Scientific alpha Actinin 1 Polyclonal Antibody
Human, Mouse, Rat에 반응하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. Recombinant Human ACTN1 단백질을 면역원으로 제작. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer와 glycerol 함유. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ACTN1. Recombinant protein control fragment (Product #RP-88918). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789394 |
Product Specific Information
Immunogen sequence:
SAKEGLLLWC QRKTAPYKNV NIQNFHISWK DGLGFCALIH RHRPELIDYG KLRKDDPLTN LNTAFDVAEK YLDIPKMLDA EDIVGTARPD EKAIMTYVSS FYHAFSGAQK AETAANRICK V
Target Information
Alpha Actinin 1 belongs to the spectrin gene superfamily, a diverse group of cytoskeletal proteins including alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in various cell types.
In nonmuscle cells, it is found along microfilament bundles and adherens-type junctions, binding actin to the membrane. In muscle cells, it localizes to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
This gene encodes a nonmuscle, cytoskeletal alpha actinin isoform and maps to the same site as the erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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