Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), Brilliant Ultra Violet 615, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-30S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with mouse CD24 (Heat Stable Antigen, HSA), a 35–50 kDa GPI-anchored surface molecule expressed on erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells. CD24 expression varies with cell lineage and glycosylation status, aiding in differentiation of B-cell development stages in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimal performance requires titration.

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is excited by a 355 nm UV laser. Ensure cytometer compatibility.

Staining Panel Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD24 is a GPI-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells in bone marrow to mature surface Ig-positive B-cells. Expression is low or absent in plasma cells. CD24 is also found in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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