Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and acts as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression as well as cytotoxic activity of CD3-activated T cells.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (1.0 µg) per test. A test is defined as the amount of antibody that will stain a cell sample in 100 µL final volume. Cell number may range from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is suitable for cytometers equipped with an ultraviolet (355 nm) laser. Ensure instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and compensation.

Excitation / Emission:
350 nm / 615 nm (Ultraviolet Laser)

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein. It functions as a co-stimulatory receptor, binding CD80 and CD86 on antigen-presenting cells to provide a critical second activation signal for T cells. CD28 prevents T cells from entering an anergic state or premature apoptosis. It is expressed on most peripheral blood T lymphocytes, with expression patterns varying across thymocyte maturation stages. CD28 dysfunction is associated with diseases such as mycosis fungoides and Sezary’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지