Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Ultra Violet 615, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II, associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.
• Fluorochrome: Brilliant Ultra Violet™ 615 (BUV615), tandem dye emitting at 615 nm for UV (355 nm) laser cytometers.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a 95 kDa transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on all peripheral B cells (kappa or lambda light chain positive) and some myeloid leukemia cells. CD19 acts as a co-receptor with the B cell receptor (BCR), lowering activation threshold and amplifying signaling. It is a key regulator of normal and malignant B cell proliferation. Mutations cause hypogammaglobulinemia; overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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