Thermo Fisher Scientific Factor VIII Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

-

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human Factor VIII.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

13.8 mg/mL

Purification

SDS-PAGE

Storage buffer

50% water with 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2262541

Product Specific Information

PA1-43045 detects Factor VIII in human samples.

PA1-43045 has successfully been used in Western blot and ELISA applications.

PA1-43045 was derived from human factor VIII.

The molecular weight of PA1-43045 is 150 kDa.

Target Information

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.