Thermo Fisher Scientific Factor VIII Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 5–10 µg/mL

Inhibition Assays (IA)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

PA1-43045 detects Factor VIII in human samples.
PA1-43045 has successfully been used in Western blot and ELISA applications.
PA1-43045 was derived from human Factor VIII.
The molecular weight of PA1-43045 is approximately 150 kDa.

Target Information

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation.
Factor VIII acts as a cofactor for factor IXa, which, in the presence of Ca²⁺ and phospholipids, converts factor X to the activated form Xa.
This gene produces two alternatively spliced transcripts:

• Transcript variant 1: Encodes a large glycoprotein (isoform a) that circulates in plasma and associates with von Willebrand factor in a noncovalent complex.
• Transcript variant 2: Encodes a smaller protein (isoform b) consisting primarily of the phospholipid binding domain, essential for coagulant activity.

Defects in this gene result in hemophilia A, a common recessive X-linked coagulation disorder.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.