Thermo Fisher Scientific c-Myc Chimeric Recombinant Rat Monoclonal Antibody (9E10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Specificity: Epitope sequence EQKLISEEDL located in aa 410–419 of human c-myc protein.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with cell proliferation and cycle regulation.
c-Myc is essential for vasculogenesis and angiogenesis during tumor development. Deregulated c-Myc expression can induce apoptosis in various cell types.
Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to amino- or carboxy-termini of target proteins.
Amplification of c-Myc has been observed in lung, breast, and colon carcinomas, while N-Myc amplification is common in neuroblastomas.
Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is described in Burkitt’s lymphoma and other B-cell lymphoproliferative conditions.
Aberrant expression of c-myc occurs in various tumor types including colorectal, gastric, hepatic, ovarian, mammary, pulmonary, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.