Thermo Fisher Scientific PKC alpha Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Positive test controls: NIH/3T3, Jurkat, C6, SH-SY5Y, Mouse brain, Rat brain
• Localization: Cell membrane, Cytoplasm, Mitochondrion membrane, Nucleus, Peripheral membrane protein

Immunogen sequence:
MTKHPAKRLG CGPEGERDVR EHAFFRRIDW EKLENREIQP PFKPKVCGKG AENFDKFFTR GQPVLTPPDQ LVIANIDQSD FEGFSYVNPQ FVHPILQSAV

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent.
Protein kinase C (PKC) can be activated by calcium and diacylglycerol, phosphorylating diverse protein targets and participating in various cellular signaling pathways.
PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.
This kinase plays roles in cell adhesion, transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest it regulates cardiac contractility and Ca²⁺ handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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