Thermo Fisher Scientific MDM2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Positive test controls include: A-549, Mouse lung, Mouse thymus, Rat lung.
The target is usually found in the following locations: Cytoplasm, Nucleus, nucleolus, nucleoplasm.

Immunogen sequence:
CVIREICCER SSSSESTGTP SNPDLDAGVS EHSGDWLDQD SVSDQFSVEF EVESLDSEDY SLSEEGQELS DEDDEVYQVT VYQAGESDTD SFEEDPEISL ADYWKCTSCN EMNPPLPSHC NRCWALRENW LPEDKGKDKG EISEKAKLEN STQAEEGFDV PDCKKTIVND SRESCVEEND DKITQASQSQ ESEDYSQPST SSSIIYSSQE DVKEFEREET QDKEESVESS L

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of p53 stability.
Located on chromosome 12q, MDM2 phosphorylation at Ser166 and Ser186 by Akt enhances its interaction with p300, promoting ubiquitination and degradation of p53.
Phosphorylation also prevents MDM2 binding to p19ARF, further increasing p53 degradation.
Overexpression of MDM2 can inactivate p53 and reduce its tumor suppressor function.
Additionally, MDM2 influences apoptosis and cell cycle regulation, and is overexpressed in various human cancers, including soft tissue carcinomas and breast cancer.
MDM2 also interacts with other proteins such as retinoblastoma 1 and ribosomal protein L5.
More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.