Thermo Fisher Scientific Calpain S1 Monoclonal Antibody (SP81)
Calpain S1 단백질을 인식하는 SP81 클론의 Rabbit Monoclonal 항체입니다. IHC(P) 및 Flow Cytometry에 사용 가능하며, Human, Mouse, Rat에 반응합니다. Protein A/G로 정제된 액상 형태로 제공되며, PBS buffer(1% BSA) 내 보관됩니다. 연구용 전용 제품입니다.
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Thermo Fisher Scientific Calpain S1 Monoclonal Antibody (SP81)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Flow Cytometry (Flow) | 1:20 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP81 |
| Immunogen | Synthetic peptide derived from internal region of human calpain protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537845 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.
Target Information
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases.
They are implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress.
Calpain I and II are heterodimeric enzymes with distinct large subunits and a common small subunit, each encoded by different genes.
Calpain S1 is the small subunit common to both calpain I and II and is associated with myotonic dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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