Thermo Fisher Scientific IL-4 Monoclonal Antibody (BVD6-24G2), PE-Cyanine7, eBioscience
마우스 IL-4를 인식하는 단클론 항체로, PE-Cyanine7으로 표지되어 흐름세포분석에 최적화됨. Th2 세포 유래 IL-4 검출에 사용. 5 µL/test로 사용 권장. 광민감성이 있으므로 암조건 보관 필요.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 20 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BVD6-24G2 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569/780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1659723 |
Available Formats
Product Specific Information
Description:
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.
Applications Reported:
This antibody is reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested for intracellular staining and flow cytometric analysis of restimulated mouse splenocyte cultures. Recommended use: 5 µL (0.5 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation; protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
Clone-specific performance should be determined empirically.
Excitation/Emission:
488–561 nm / 775 nm; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin-4 receptor.
IL-4 has a broad range of biological actions and shares overlapping functions with IL-13 through receptor binding. STAT6 mediates IL-4–induced immune regulatory signaling.
The IL-4 gene is located on chromosome 5q within a cytokine gene cluster (IL-3, IL-5, IL-13, CSF2) and is closely linked to IL-13.
IL-4, IL-5, and IL-13 are co-regulated by long-range regulatory elements spanning approximately 120 kb.
IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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