Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (B2D), PE-Cyanine7, eBioscience
마우스 ROR gamma(t) 단백질을 인식하는 B2D 단클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. Flow cytometry 분석에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 4°C 암조건에서 보관하며, 광산화에 민감하므로 빛 차단 필요.
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Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (B2D), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B2D |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784670 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein, a member of the retinoic acid-related orphan receptor (ROR) family. ROR gamma is expressed in various tissues including liver, lung, muscle, heart, and kidney. The isoform ROR gamma t is expressed exclusively in lymphoid cells such as CD4+CD8+ thymocytes, Th17 cells, and lymphoid tissue inducer (Lti) cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested with mouse thymocytes; ≤ 0.5 µg/test (100 µL final volume). Optimize antibody titration for best assay performance.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57).
Excitation/Emission: 488–561 nm / 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
ROR gamma (NR1 Thyroid Hormone-Like receptor) influences thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. It promotes thymocyte survival via Bcl-x(L) activation and is essential for lymph node and Peyer’s patch development. The thymus-specific isoform ROR gamma(t) inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds to GGTCA motifs preceded by AT-rich sequences.
Expression documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. ESTs identified from various human tissues including cancerous and normal samples.
Note: ROR gamma t (RORgt) is an isoform of RORC.
For Research Use Only. Not for diagnostic procedures or resale without authorization.
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