Thermo Fisher Scientific HP1 alpha Polyclonal Antibody
HP1 alpha 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, Flow cytometry 및 IP에 사용 가능. 인간, 마우스, 원숭이, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제, -20°C 보관.
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Thermo Fisher Scientific HP1 alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 1:25 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of human HP1alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 120 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10987489 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with HP1 beta or HP1 gamma proteins.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, which act as heterochromatic adaptor molecules involved in gene silencing and chromatin structure.
High-affinity recognition of methylated histone H3 by HP1 requires a functional chromodomain, serving as a specific interaction motif for the methyl epitope on lysine-9. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-mutant mouse fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-17441_HP1_alpha_P45973-1_Rabbit.svg, PA5-17441_HP1_alpha_P45973-1_Rabbit_PDP.jpeg)
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