Thermo Fisher Scientific AMIGO1 Monoclonal Antibody (L86/33)
AMIGO1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC에 사용 가능. Human, Mouse, Rat 반응성. 단백질 G로 정제된 1 mg/mL 액상 항체로, PBS/glycerol buffer에 보관. 신경세포 성장 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L86/33 |
| Immunogen | Fusion protein amino acids 394–492 (cytoplasmic C-terminus) of human AMIGO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735110 |
Additional Formats
- APC (Cat. MA5-45619)
- FITC (Cat. MA5-45620)
- PE (Cat. MA5-45622)
- PerCP (Cat. MA5-45621)
- Custom conjugation available upon request
Product Specific Information
- 1 µg/mL of MA5-27611 detects AMIGO-1 in 20 µg of rat brain membrane lysate using colorimetric immunoblot analysis with goat anti-mouse IgG:HRP as secondary antibody.
- Detects bands at approximately 60–80 kDa depending on maturity/glycosylation.
- Formerly sold as clone S86-36.
Target Information
AMIGO1 promotes growth and fasciculation of neurites from cultured hippocampal neurons. It may be involved in myelination and regeneration of neural axons, contributing to neural plasticity in the adult nervous system. AMIGO1 mediates cell-cell interactions and participates in signal transduction through its intracellular domain. When assembled with KCNB1, it modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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