Thermo Fisher Scientific IKK beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

ELISA (ELISA)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

-

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Product Specifications

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH- which corresponds to the highly conserved C-terminus region of the human protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2295677

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.