Thermo Fisher Scientific ABCA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:400

-

Immunoprecipitation (IP)

1:10-1:500

-

ChIP assay (ChIP)

Assay-dependent

-

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mustelid, Mouse, Pig, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide corresponding to amino acids 1100-1300 of Human ABCA 1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ABCA1,uniProtId:O95477-1,ncbiNodeId:9606,antigenRange:1100-1300,antigenLength:2261,antigenImageFileName:PA1-32129_ABCA1_O95477-1_Rabbit.svg,antigenImageFileNamePDP:PA1-32129_ABCA1_O95477-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2257654

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.