Norgen Stool Total RNA Purification Kit Dx

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브랜드: Norgen
카테고리: Other Organics
판매단위: pk

#Norgen #Reagents #RNA Reagents #R

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Norgen · Norgen Stool Total RNA Purification Kit Dx

Norgen Stool Total RNA Purification Kit Dx

SKU: Dx49500
A convenient and rapid method to purify total RNA from small amounts of stool samples for in vitro diagnostic use.

Features and Benefits

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746
Ideal for use in in vitro diagnostic workflows
Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
Isolate full diversity of RNA from large RNA down to small and microRNAs
Eliminates PCR inhibitors including humic acids
High quality RNA for sensitive downstream applications

Product Description

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed, including animal fecal samples, manure, and samples collected using Norgen’s Stool Nucleic Acid Collection and Transport Devices Dx (Cat. Dx45660).
It removes humic acids using simple spin column procedures and includes bead tubes for effective homogenization. The kit purifies all sizes of RNA—from large mRNA and ribosomal RNA to microRNA and siRNA. Both host and microbial RNA are recovered. The protocol avoids phenol or chloroform, ensuring a user-friendly and high-throughput workflow. Purified RNA is suitable for downstream applications such as real-time PCR and RT-PCR for gene expression analysis.
Processing time: approximately 30 minutes.

NOTE: This product is not available for sale in the United States.

Supporting Data

Figure 1. High yields of stool total RNA were purified from 200 mg of human stool using Norgen’s Stool RNA Purification Kit Dx and a leading competitor’s kit, in triplicate.
For analysis, 7.5 µL of each 75 µL elution was loaded on a 1.2% 1x MOPS formaldehyde-agarose gel. Norgen’s kit showed higher RNA yield from 200 mg of human infant stool.

Norgen	Competitor

Figure 2. Yield and Quality of Purified Stool RNA.
Total RNA was isolated from 200 mg of human stool using Norgen’s Stool RNA Purification Kit Dx and a competitor kit. Yield and purity were compared using NanoVue Plus™ A260:A280 and A260:A230 ratios.
Norgen’s kit produced RNA with high A260:A230 ratios (1.8–2.2 range), while the competitor’s kit showed low A260:A230 (<0.20). Average yield: Norgen 14.58 µg vs Competitor 12.26 µg.

Norgen	Competitor
(이미지 내 젤 밴드)	(이미지 내 젤 밴드)

Figure 3. Detection of Bacterial Stool RNA using 16S Primers.
Total stool RNA was isolated from 200 mg human stool samples using both kits. Five microliters of purified RNA was used for reverse transcription and qPCR using bacterial 16S primers. Norgen’s kit outperformed the competitor by ~1.5 Ct values, indicating higher RNA yield and quality.

Kit Specifications

Specification	Description
Maximum Stool Input	200 mg
Type of Stool Processed	Frozen and fresh stool
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Time to Complete 10 Purifications	30 minutes

Storage Conditions and Product Stability
All solutions should be tightly sealed and stored at room temperature. Stable for 2 years after shipment.

Component	Cat. Dx49500 (50 preps)
Lysis Solution	60 mL
Wash Solution	22 mL
Elution Buffer	6 mL
Bead Tubes	50
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Documentation

Protocol: (Dx49500) Stool Total RNA Purification Kit Dx - Protocol (50 prep)
Application Note: Purification and Detection of Bacterial RNA From Preserved Stool Samples
SDS: Dx49500 - Stool Total RNA Purification Kit Dx - SDS
Flyer: Product Flyer

Citations

Title	Journal	Authors
The role of whole-grain barley on human fecal microbiota and metabolome	Applied and Environmental Microbiology, 2015	Maria De Angelis et al.
Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives	Food Microbiology, 2015	Maria De Angelis et al.
Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation	BMC Genomics, 2015	Michael Reck et al.
Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets	PLOS ONE, 2015	Ilario Ferrocino et al.
Modulation of the Intestinal Microbiota Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour	Journal of Agriculture and Food Chemistry, 2015	Hyunsook Kim et al.
Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN)	Microbiota and Metabolome of IgA Nephropathy Patients, 2014	Maria De Angelis et al.