Norgen Hepatitis C Virus (HCV) TaqMan RT-PCR Detection Kits

Norgen Hepatitis C Virus (HCV) TaqMan RT-PCR Detection Kits

SKU: TM37610

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Features and Benefits

• Detection kits for HCV
• Available in TaqMan format for analysis

Hepatitis C virus infection is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC) worldwide. Neither a vaccine nor an effective treatment is available for HCV. Pegylated interferon-α (PEG-IFN-α), combined with ribavirin, is the current standard therapy. Its efficiency ranges between 20–80%, depending on the HCV genotype. Unfortunately, many HCV-infected patients do not respond to or tolerate IFN-based therapy, leading to an expected increase in HCC cases over the next decades. The infection is often asymptomatic, evades immune responses, and remains undetected until severe liver damage develops.

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TaqMan PCR Kit Options

HCV TaqMan RT-PCR Kit (100 reactions)

• Ready-to-use format including Master Mix, target, and PCR control
• Specific Primer and Probe mix for HCV detection
• Positive and negative controls included

HCV TaqMan RT-PCR Probe/Primer Set and Controls (100 reactions)

• Specific Primer/Probe mix and Positive Control for HCV
• Nuclease-free water included
• Compatible with Norgen’s RT-PCR Master Mix (#28113) or user-supplied mix

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

Figure 1. Positive Result

Both PCR signals above the baseline from FAM and HEX channels indicate successful PCR amplification.

그래프 정보 (OCR 텍스트):

• 상단 그래프: Amplification – FAM channel, X축: Cycles, Y축: RFU
• 하단 그래프: Amplification – HEX channel, X축: Cycles, Y축: RFU
• 두 그래프 모두 증폭 곡선을 나타냄 (FAM: 파란색, HEX: 초록색)

Figure 2. Negative Result

No target RNA was detected in FAM channel, while HEX channel shows amplification indicating valid PCR.

그래프 정보 (OCR 텍스트):

• 상단: Amplification – FAM channel, 평평한 곡선
• 하단: Amplification – HEX channel, 약 25 사이클 이후 급격한 증폭
• X축: Cycles (0–40), Y축: RFU

Figure 3. Inhibition Result

No signal from both FAM and HEX channels detected. Suggested to repeat RNA purification.

그래프 정보 (OCR 텍스트):

• 상단: Amplification – FAM channel, X축: Cycles, Y축: RFU
• 하단: Amplification – HEX channel, X축: Cycles, Y축: RFU
• 두 그래프 모두 증폭 신호 없음 (억제 상태)

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Storage Conditions and Product Stability

• All kit components stable for 1 year after production without performance reduction.
• Store all components at -20°C upon arrival.

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Documentation

Protocols

• (TM37650) HCV TaqMan RT-PCR Kit - Protocol (100 rxns)
• (TM37610) HCV TaqMan Probe/Primer and Control Set - Protocol (100 rxns)

Safety Data Sheets (SDS)

• TM37650 - HCV TaqMan RT-PCR Detection Kit - SDS
• TM37610 - HCV TaqMan RT-PCR Detection Kit - SDS

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Related Products

• HCV TaqMan RT-PCR Kit (100 reactions)
• Plasma/Serum RNA Purification Kit

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