Thermo Fisher Scientific AKT2 Monoclonal Antibody (OTI4H7), TrueMAB
AKT2 단백질을 인식하는 Mouse Monoclonal Antibody로 Western blot, IHC, ICC/IF, Flow Cytometry에 적합. 인간 시료에 반응하며, Affinity chromatography로 정제된 Lyophilized 형태. 재구성 시 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4H7 |
| Immunogen | Full length human recombinant protein of human AKT2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
AKT2 is a phosphoinositide-dependent serine/threonine protein kinase enriched in insulin-responsive tissues and involved in metabolic actions of insulin.
It functions as a key transducer in multiple cellular signaling pathways activated by growth factor receptors that stimulate phosphatidylinositol 3-kinase (PI3K).
AKT2 amplification and overexpression have been observed in certain human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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