
Norgen Parvo B19 TaqMan PCR Kit Dx
Parvovirus B19 검출용 TaqMan PCR 키트로, CE-IVDD 인증을 받은 체외진단용 제품입니다. 24회 반응용으로 구성되어 있으며, PCR 억제 모니터링 및 품질 검증용 내부 컨트롤을 포함합니다. -20°C 보관이 필요하며, 빠르고 정확한 진단을 위한 Ready-to-use 포맷을 제공합니다.
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Norgen Parvo B19 TaqMan PCR Kit Dx
SKU: DxTM39600
Features and Benefits
- Detection kits for Parvovirus B19
- CE-IVDD marked in accordance with European Commission Directive 98/79/EC
- Research version available
- Available in TaqMan format for analysis
Product Description
Parvovirus B19 was the first known human virus in the family of Parvoviruses. It is a non-enveloped, icosahedral virus containing a single-stranded linear DNA genome. Classified as an erythrovirus, it invades red blood cell precursors in bone marrow.
B19 causes a childhood rash called fifth disease (erythema infectiosum), commonly known as “slapped cheek” syndrome. Transmission occurs primarily via respiratory droplets, with some blood-borne cases reported.
In pregnant women, infection may cause hydrops fetalis and fetal anemia, sometimes leading to miscarriage or stillbirth. Rapid diagnostic methods are essential due to the severity of infection.
Kit Format
Parvo B19 TaqMan PCR Kit, 24 reactions
- Ready-to-use format including Master Mix and PCR control
- Specific Primer and Probe mix for pathogen detection
- Positive and negative controls included
- Validates quality and monitors PCR inhibition
Supporting Data
Figure 1: Example of TaqMan PCR Positive Result
Both PCR signals above the baseline from FAM and HEX channels indicate successful PCR amplification.
Amplification Graphs (OCR extracted):
| 그래프 위치 | 제목 | 채널명 | X축 | Y축 |
|--------------|--------|-----------|------|------|
| 상단 | Amplification | FAM channel | Cycles (10~40) | RFU (-100~500) |
| 하단 | Amplification | HEX channel | Cycles (10~40) | RFU (0~900) |
Figure 2: Example of TaqMan PCR Negative Result
No target DNA detected in FAM channel; HEX amplification confirms successful PCR control.
Amplification Graphs (OCR extracted):
| 그래프 위치 | 제목 | 채널명 | X축 | Y축 |
|--------------|--------|-----------|------|------|
| 상단 | Amplification | FAM channel | Cycles (0~40) | RFU (-100~400) |
| 하단 | Amplification | HEX channel | Cycles (0~40) | RFU (-100~900) |
Figure 3: Example of TaqMan PCR Inhibition Result
No signal detected from both FAM and HEX channels. Repeat sample preparation using recommended DNA purification kit.
Amplification Graphs (OCR extracted):
| 그래프 위치 | 제목 | 채널명 | X축 | Y축 |
|--------------|--------|-----------|------|------|
| 상단 | Amplification | FAM channel | Cycles (0~40) | RFU (-100~900) |
| 하단 | Amplification | HEX channel | Cycles (0~40) | RFU (-100~900) |
Storage Conditions
- Shipped on dry ice
- Store all components at -20°C upon arrival
- Avoid repeated thawing/freezing (>3x) of Master Mix and Positive Control
- For intermittent use, freeze reagents in aliquots
Kit Components
| Component | Cat. DxTM39600 (24 rxns) |
|---|---|
| MDx TaqMan 2X PCR Master Mix Dx | 550 µL |
| Parvo B19 Primer & Probe Mix Dx | 2 × 70 µL |
| Parvo B19 Positive Control Dx (200,000 copies/μL) | 50 µL |
| Nuclease-Free Water | 1.25 mL |
| Product Insert | 1 |
Documentation
Protocols
(DxTM39600) Parvo B19 TaqMan PCR Kit Dx - Protocol (24 rxns)
Safety Data Sheets
DxTM39600 - Parvovirus 19 TaqMan PCR Detection Kit Dx - SDS
Related Products
- Parvo B19 Detection Kit (100 reactions)
- Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit
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