Thermo Fisher Scientific Human IL-6 Recombinant Protein
인간 IL-6 재조합 단백질로 염증 및 면역 반응 연구에 적합합니다. E. coli 발현 시스템에서 생산되며 순도 95% 이상입니다. 기능적 활성 검증 완료, 다양한 분석(ELISA, WB, IHC)에 활용 가능. 동결건조 형태로 안정적 보관 가능.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- View 1 publication
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM |
| Molecular Weight | 21.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 < 20.0 pg/mL; determined by dose-dependent proliferation of mouse B9 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge the vial briefly before opening to bring contents to the bottom.
Reconstitute the lyophilized protein in 100 mM acetic acid to a concentration of 0.1–1.0 mg/mL to regain full activity.
Apportion the reconstituted protein into working aliquots and store at ≤ -20°C.
Make further dilutions in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.Storage:
Store the lyophilized protein at 2–8°C, preferably desiccated.
Upon reconstitution, apportion into working aliquots and store at ≤ -20°C (not in a frost-free freezer).
Avoid repeated freeze-thaw cycles.
Target Information
IL-6 is a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen, inducing fever in autoimmune or infectious conditions. Expression occurs at sites of acute and chronic inflammation, where it activates transcriptional responses via the interleukin 6 receptor alpha.
This gene is associated with various inflammatory diseases, including diabetes mellitus, systemic juvenile rheumatoid arthritis, Kaposi sarcoma, and rheumatoid arthritis. Elevated IL-6 levels are also observed in viral infections such as COVID-19 (SARS-CoV-2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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