Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet 711

Applications and Tested Dilution

• Flow Cytometry (Flow): Assay-Dependent
• Control (Ctrl): Assay-Dependent

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421, 480, 605, 650, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

• Description: Monoclonal mouse IgG1 kappa immunoglobulin, useful as an isotype control.
• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Validated in flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells; use at same concentration as experimental antibody.
• Fluorochrome: Brilliant Violet™ 711 (BV711) emits at 713 nm, optimized for violet (405 nm) laser-equipped cytometers.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize polymer dye interactions.
• Light Sensitivity: Protect vial and stained samples from light to prevent photo-induced oxidation.
• Fixation Compatibility: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333). Not compatible with methanol-based fixation.
• Excitation / Emission / Laser: 407 nm / 713 nm / Violet Laser.
• Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigens.
It is the most abundant IgG subtype in serum and plays a key role in adaptive immunity, including:

• Opsonization
• Complement activation
• Antibody-dependent cell-mediated cytotoxicity
• Regulation of immune responses (T cell activation, B cell inhibition)

Widely used in:

• Research and diagnostic applications (ELISA, Western blotting, immunohistochemistry)
• Therapeutic development (immunotherapy, vaccine research)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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