Thermo Fisher Scientific SETD8 Monoclonal Antibody (OTI2F5), TrueMAB
SETD8 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB 및 IHC(P) 실험에 적합합니다. 인간 SETD8 전체 길이 재조합 단백질로 면역화되었습니다. 동결건조 형태이며, PBS buffer와 trehalose를 포함합니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F5 |
| Immunogen | Full length human recombinant protein of human SETD8 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SETD8 is a histone methyltransferase that specifically monomethylates Lys-20 of histone H4.
H4 Lys-20 monomethylation is enriched during mitosis and acts as a tag for epigenetic transcriptional repression.
SETD8 functions mainly in euchromatin regions, contributing to gene silencing, chromosome condensation, and proper cytokinesis.
Nucleosomes are preferred substrates compared to free histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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