Thermo Fisher Scientific PTPRC Monoclonal Antibody (OTI3B9), TrueMAB
Human PTPRC(CD45) 단백질을 인식하는 Mouse IgG1 모노클로날 항체로, WB, IHC(P), Flow Cytometry에 적합합니다. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose)로 안정화되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:8000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B9 |
| Immunogen | Full length human recombinant protein of human PTPRC produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, accounting for about 10% of surface proteins in lymphocytes.
It is absent in non-hematopoietic cell lines and tissues. CD45 plays a crucial role in lymphocyte development and antigen signaling, regulating Src-family kinases.
Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains.
CD45RA, one isoform, shows restricted expression among lymphoid cell subtypes.
CD45 regulates T- and B-cell receptor signaling, suppresses JAK kinases, and modulates integrin-mediated pathways, apoptosis, and various immune functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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